方法论 / 技术路线

Therapeutic siRNA Design Pipeline

End-to-end siRNA discovery from target nomination to LNP-formulated in vivo delivery.

完整 Prompt

Create a therapeutic siRNA design pipeline diagram from target nomination to LNP-formulated in vivo delivery.

Layout:
- Sequential stages: disease target nomination, transcript selection, siRNA candidate design, off-target filtering, chemical modification, in vitro potency screen, LNP formulation, in vivo biodistribution and knockdown.
- Show go / no-go gates after off-target filtering, potency screen, and in vivo validation.
- Include small icons for mRNA, siRNA duplex, hepatocyte or target cell, lipid nanoparticle, and qPCR readout.
- Add metric callouts: seed off-target score, IC50, serum stability, knockdown percentage.

Style:
- Biomedical pipeline diagram on white background.
- Navy text, teal forward arrows, coral rejection paths, amber metric callouts.
- Clean vector style with readable labels and consistent spacing.
- Suitable for RNA therapeutics papers and translational research presentations.

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适用场景

For RNA therapeutics, drug-discovery proposals and translational pipeline figures.

变体

Add a non-human-primate stage

Insert a Stage 5b "NHP Pharmacokinetics" between LNP Formulation and In Vivo Validation, showing biodistribution and immune response checks before mouse efficacy work.

使用建议

Number stages 1-6 — readers parse pipelines top-down by number first.
Annotate each stage's output, not just its action. "Knockdown leads" beats "screen".
Keep arrow labels short. Pipeline figures fail when arrows carry sentences.

常见问题

Can I show the failure rate at each stage?

Add a side-bar funnel showing candidate count at each stage (e.g., 10000 -> 200 -> 20 -> 5 -> 2 -> 1). Place it to the right of the main pipeline.